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Fig. 2 | Molecular Medicine

Fig. 2

From: NK cell frequencies, function and correlates to vaccine outcome in BNT162b2 mRNA anti-SARS-CoV-2 vaccinated healthy and immunocompromised individuals

Fig. 2

Manual and automated analysis of NK cells in healthy study subjects. A UMAP plots from manual gating analysis on concatenated files of total NK cells in healthy study subjects grouped together and separated by Days 0, 10, 21 and 35. B UMAP of CD56bright, CD56brightCD16pos, CD56dim and CD56dimCD16neg within pooled NK cells from healthy controls at all timepoints. C Expression of the indicated functional and phenotypic markers on total NK cells in the healthy control group represented in UMAP plots (upper panel) and corresponding histograms at Days 0, 10, 21 and 35 (lower panel). D Automated analysis showing UMAP of total NK cells depicting selected PhenoGraph overlaid clusters. E Distribution of the different timepoints analyzed (Days 0, 10, 21 and 35) within each PhenoGraph cluster. F Percentage of 20 PhenoGraph clusters within total NK cells at Days 0, 10, 21 and 35. G Heatmap of the mean fluorescence intensity (MFI) of the phenotypic markers used to characterize NK cells across PhenoGraph clusters at Days 0, 10, 21 and 35 (quantification of median expression values as column-z score). H Flow cytometry histograms showing expression of Perforin within the selected clusters. Healthy individuals (Day 0 n = 37, Day 10 n = 38, Day 21 n = 36, Day 35 n = 37)

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