Fig. 3

NK cell functional status at baseline and its correlation to anti-SARS-CoV-2 Ab titers. Functional characterization of total NK cells from healthy study subjects and select patient groups with immunodeficiency disorders receiving two doses of the BNT162b2 mRNA vaccine according to label. Samples at Days 0, 10, 21 and 35 show the frequencies of IFNγ-, TNF-, Granzyme B-, and CD107a-positive NK cells after 24 h of IL-12 (10 ng/ml) and IL-18 (100 ng/ml) stimulation (upper panel). Representative flow cytometry plots of functional markers expressed on NK cells (lower panel). Healthy controls (Day 0 n = 30, Day 10 n = 30, Day 21 n = 26, Day 35 n = 35), PLWH (Day 0 n = 25, Day 10 n = 24, Day 21 n = 24, Day 35 n = 23), PID (Day 0 n = 5, Day 10 n = 8, Day 21 n = 7, Day 35 n = 7), SOT (Day 0 n = 5, Day 10 n = 5, Day 21 n = 5, Day 35 n = 4). Statistical analysis was performed using a Kruskal–Wallis test followed by a Dunn’s multiple comparisons test. No statistical significance difference was observed within each respective group (i.e., when comparing days 0, 10, 21 and 35)