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Fig. 2 | Molecular Medicine

Fig. 2

From: Elevated RIF1 participates in the epigenetic abnormalities of zygotes by regulating histone modifications on MuERV-L in obese mice

Fig. 2

Development potential in oocytes and preimplantation embryos of HFD mice. A Developmental status captures of oocytes and embryos from the HFD and CD groups. Red asterisks indicate abnormal oocytes or embryos. Bottom: Statistical results of developmental status (oocytes, n = 90 for CD and n = 77 for HFD; 2-cell embryos, n = 80 for CD and n = 54 for HFD; blastocysts, n = 53 for CD and n = 23 for HFD). B Reactive oxygen species (ROS) of MII oocytes stained with dihydroethidium from the HFD and CD groups. The red fluorescence intensity represents the level of ROS. Bottom: The statistical results are shown between the HFD and CD groups. C Mitochondrial membrane potential (MMP) of MII oocytes stained with JC-1 from the HFD and CD groups. The ratios of the red/green fluorescence intensity indicated the levels of MMP, and Bottom: The statistical results are shown between the HFD and CD groups. D Representative immunofluorescence results of SIRT3 and PPARγ in oocytes and blastocysts from the HFD and CD groups. E Western blot analysis of SIRT3 and PPARγ in oocytes from the HFD and CD groups. F: qPCR results of SIRT3 and PPARγ from oocytes to blastocysts between the HFD and CD groups. Throughout, n = 20 in each group for IF analysis and n = 3 in each group for WB or qPCR analysis. Data are presented as means ± SD. P values are calculated by Student’s t test. *p < 0.05, **p < 0.01, ***p < 0.001. ns indicates not significant. Scale bars are shown in the lower right corner of the captures. HFD high-fat diet, CD control diet, BC blastocyst

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