Fig. 4
From: Lansoprazole-induced osteoporosis via the IP3R- and SOCE-mediated calcium signaling pathways
Calcium regulation in MC3T3-E1 cells was observed by confocal microscopy. A Representative confocal images (40 ×): After LPZ administration, intracellular calcium fluorescence increased significantly. B Quantification of Fluo-3 fluorescence: Ca2+ fluorescence was slightly higher with calcium than without calcium. C Area under the curve of Fluo-3 fluorescence: There was no significant difference in the area under the curve of calcium fluorescence with or without calcium. D Fluo-3 fluorescence after preincubation with TG (n = 3): After preincubation with TG to deplete calcium stores, LPZ did not cause a significant increase in cellular calcium fluorescence. E The viability of MC3T3-E1 cells after preincubation with BAPTA/AM and LPZ treatment with CCK-8: BAPTA prevented the increase in Ca2+ and could effectively protect cell viability