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Fig. 2 | Molecular Medicine

Fig. 2

From: Phosphorylation of BCL2 at the Ser70 site mediates RANKL-induced osteoclast precursor autophagy and osteoclastogenesis

Fig. 2

Tg-hRANKL mice had stronger BCL2 phosphorylation capacity at S70, not S87, in OCPs. A Representative 3D micro-CT reconstructed images of the tibiae from Tg-hRANKL mice and control mice in the same nest (WT mice) showing bone mass and bone microstructure (N = 6/group). Scale bar, 2 mm or 1 mm. B Representative H&E-stained tibial sections from each group. Scale bar, 20 μm. C Representative TRAP-stained tibial sections from each group (Red arrows indicate TRAP+ cells). Scale bar, 5 μm. D Tibial sections were stained with red and green fluorochromes for p-BCL2 (S70 or S87) and RANK, respectively, and observed using fluorescence microscopy. The overlapping staining of p-BCL2 and RANK are indicated with red arrows (yellow fluorescence). Scale bar, 2.5 μm. E Representative fluorescent images of p-BCL2 (S70 or S87) (red arrows) in bone marrow RANK+ CSF1R+ cells sorted by FACS. Scale bar, 20 μm. FK The trabecular bone parameters, including BMD, BV/TV, BS/BV, Tb.Th, Tb.N, and Tb.Sp were analysed using micro-CT. L The trabecular bone parameter, Tb.Ar, was analysed using H&E staining and IPP system. M The number of osteoclasts per millimeter of trabecular bone surface was counted. (N) The percentages of p-BCL2 (S70)-positive cells or p-BCL2 (S87)-positive cells in E (30 cells per field, N = 5). The experiments were replicated at least three times. Data are presented as the mean ± SEM. ***P < 0.001, **P < 0.01. ns not significant, WT control mice in the same nest

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