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Fig. 1 | Molecular Medicine

Fig. 1

From: Activation of dopamine receptor D1 promotes osteogenic differentiation and reduces glucocorticoid-induced bone loss by upregulating the ERK1/2 signaling pathway

Fig. 1

Dex inhibited the differentiation of osteoblasts and reduced D1R expression in vitro. A CCK-8 analysis of Dex-induced MC3T3-E1 cells. B Representative images showing ALP staining. Scale bar: 200 μm. C Quantitative analysis of ALP staining. n = 3 per group. NS: Not statistically significant, * p < 0.05, ** p < 0.01, vs. the Dex (−) group. D Representative images showing ARS staining. Scale bar: 200 μm. E Quantitative analysis of ARS staining. n = 3 per group. NS: Not statistically significant, * p < 0.05, ** p < 0.01, vs. the Dex (−) group. F Representative images of western blots probed with antibodies against the osteogenesis-specific proteins ALP, OSX, and Runx2 and the dopamine receptors D1–D5. GN Quantification of the protein levels. n = 3 per group. NS: Not statistically significant, * p < 0.05, ** p < 0.01, vs. the Dex (−) group

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