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Fig. 5 | Molecular Medicine

Fig. 5

From: Activation of dopamine receptor D1 promotes osteogenic differentiation and reduces glucocorticoid-induced bone loss by upregulating the ERK1/2 signaling pathway

Fig. 5

The ERK1/2 pathway was involved in the protective effect of D1R against Dex-mediated inhibition of osteoblast differentiation. A Representative images showing ALP and ARS staining. Scale bar: 200 μm. B and C Quantitative analysis of ALP and ARS staining. n = 3 per group. NS: Not statistically significant, * p < 0.05, ** p < 0.01, vs. the control group. D Representative images of western blots probed with antibodies against ALP, OSX and Runx2. E–G Quantification of ALP, OSX and Runx2 protein levels. n = 3 per group. NS: Not statistically significant, * p < 0.05, ** p < 0.01, vs. the control group. H Representative images of western blots probed with antibodies against ALP, OSX and Runx2. I–K Quantification of ALP, OSX and Runx2 protein levels in the overexpression experiment. n = 3 per group. NS: Not statistically significant, * p < 0.05, ** p < 0.01, vs. the control group

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Molecular Medicine

ISSN: 1528-3658