Fig. 2

Cell morphology, survival, and the level of pS129 α-synuclein and α-synuclein protein in rotenone-induced PD cell model transduced with NDI1. A Changes in cell morphology were examined using an inverted phase contrast microscope. (Scale bar: 100 μm). B Cell viability was examined using trypan blue exclusion staining. C, E The level of pS129 α-synuclein, α-synuclein protein was detected by western blot using pS129 α-synuclein, α-synuclein antibody with β-actin as loading control. D, F Quantitative analysis of pS129 α-synuclein, α-synuclein protein level detected by western blot. Rotenone + vector group compared with DMSO + vector group, or Rotenone + NDI1 group compared with Rotenone + vector group, or Rotenone + NDI1 group compared with DMSO + vector group, ns not significant, *P < 0.05, **: P < 0.01, ***: P < 0.001