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Fig. 5 | Molecular Medicine

Fig. 5

From: Gene therapy of yeast NDI1 on mitochondrial complex I dysfunction in rotenone-induced Parkinson’s disease models in vitro and vivo

Fig. 5

Identification of NDI1 expression in the substantia nigra and striatum of mice. A The time course of GFP expression in the SN of mice injected with AAV5-GFP was examined by western blot using GFP antibody with tubulin as loading control. W: week. B HA (NDI1) expression in the SN and striatum of mice injected with AAV5-HA-NDI1 was examined by western blot using HA antibody with GAPDH as loading control. L: left; R: right. C, D HA (NDI) expression in the SNpc or striatum of mice injected with AAV5-HA-NDI1 was examined by HA immunohistochemistry. Scale bar = 500 μm. E TH (tyrosine hydroxylase) and HA (NDI1) co-localization in dopaminergic neurons of SNpc was examined by a confocal microscopy. SNpc tissue section was co-stained with TH antibody (red, dopaminergic neurons) and HA antibody (green, HA), and counterstained with DAPI (blue, nucleus). Scale bar = 25 μm

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Molecular Medicine

ISSN: 1528-3658