Fig. 4

GRK2 mediates IL-33/ST2 signaling-induced decrease in CXCR4. A) Confocal microscopy immunofluorescence of GRK2 (green) and Hoechst (blue) in BM ILC2p at 1 h after stimulation with LPS (1 μg/ml) and/or rmIL-33 (50 ng/ml) and IL-2 (10 ng/ml), IL-7 (10 ng/ml) (n = 3 per group). B Flow cytometric analysis and C MFI of GRK2 expression in BM ILC2p after treatment with LPS and/or rmIL-33 for 1 h (n = 3 per group). D & E The percentages of CXCR4 expressing cells in total BM ILC2p after LPS (1 μg/ml) with or without rmIL-33 (50 ng/ml) or GRK2 inhibitor (150 μM) stimulation for 24 h (n = 3 per group). Data is representative of three independent in vitro experiments. Data are shown as the mean ± SEM, *P < 0.05, **P < 0.01