Fig. 1

CD248 was mainly expressed on myofibroblasts in liver fibrosis. A Sirius red staining and IHC staining of CD248 in the liver tissue of 9 heathy controls and 40 patients with liver cirrhosis. B Quantitative analysis of the data in A. C Sirius red staining and IHC staining of CD248 in the liver tissue of CCl₄-induced mice. D Quantitative analysis of the data in C. E, F qRT-PCR and western blotting analysis to show the increased expression of α-SMA, Collagen I, and CD248 in the liver tissues of CCl₄-induced mice (n = 3 in B–F). G IF staining images showing the colocalization of CD248 and α-SMA in the liver tissue of CCl₄-induced mice. Representative images are shown. Scale bar, 100 μm; **p < 0.01, ***p < 0.001. IHC immunohistochemistry, qRT-PCR quantitative real-time reverse transcription polymerase chain reaction, α-SMA alpha smooth muscle actin, IF immunofluorescence