Fig. 2
CD248 expression was upregulated on activated primary HSCs. A Flow cytometry to show the auto-fluorescent Vitamin A, which is a marker of HSCs. B, C qRT-PCR and western blotting analysis to show the increased expression of α-SMA, Collagen I and CD248 in TGF-β1-activated primary HSCs. D Flow cytometry showing the increased expression of CD248 in TGF-β1-activated primary HSCs (n = 3 in A–D). E, F qRT-PCR and western blotting analysis showing the increased expression of α-SMA, Collagen I, and CD248 in freshly isolated primary HSCs from CCl4-induced mice. G Flow cytometry showing the increased expression of CD248 in freshly isolated primary HSCs from control and CCl4-induced mice (n = 3 in E–G). Representative images are shown. *p < 0.05, **p < 0.01, ****p < 0.0001. HSC hepatic stellate cell, qRT-PCR quantitative real-time reverse transcription polymerase chain reaction, α-SMA alpha smooth muscle actin, TGF-β transforming growth factor beta