Fig. 4

Characterization of IgG78-DM1 and its bioactivity toward primary HSCs in vitro. A Flow cytometry showing the binding of IgG78-DM1 with TGF-β1-activated primary HSCs. B ELISA to show the binding affinity of IgG78-DM1 with TGF-β1 activated primary HSCs. C IF staining images showing the co-localization of IgG78-DM1 and lysosomes. Scale bar, 50 μm. D CCK-8 assay showing the effective killing of activated primary HSCs by IgG78-DM1. E Laser confocal microscopy images showing that IgG78-DM1 could destroy the assembly of α-tubulin in activated primary HSCs. Scale bar, 2 μm. F Transmission electron microscopy images showing that IgG78-DM1 could induce obvious apoptosis in activated primary HSCs. Three independent experiments were performed and analysed. Representative images are shown. DM1 Mertansine, HSC hepatic stellate cell, TGF-β transforming growth factor beta, ELISA enzyme-linked immunosorbent assay, IF immunofluorescence, CCK-8 cell counting kit-8