Fig. 2

Combined urine miRNA and SERS profiling correlates with the molecular classification of bladder cancer (BC). A Immunohistochemical staining aspect of luminal type BC. B Immunohistochemical staining aspect of basal type BC. C The two differentially expressed miRNAs by NGS analysis between luminal and basal BC. D The average SERS spectra of urine for the luminal and basal BC. E The score values of principal component (PC) 8 in luminal versus basal BC. F Loading plot of PC8. G–I Head-to-head comparison of the receiver operating characteristic (ROC) curves for the classification accuracy yielded by miRNA alone, SERS alone or the combination of the two using three supervised classification algorithms (naïve Bayes (G), logistic regression (H) and random forest (I)) for luminal and basal BC. Mean ± SD. ***p < 0.001