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Fig. 1 | Molecular Medicine

Fig. 1

From: Resveratrol ameliorates liver fibrosis induced by nonpathogenic Staphylococcus in BALB/c mice through inhibiting its growth

Fig. 1

Effect of resveratrol on liver fibrosis induced by CCl4. Balb/c male mice (6–7 weeks old) were intraperitoneally injected with olive oil or CCl4 (0.5 µL/g) to induce liver fibrosis for 4 weeks twice a week, and the mice were treated with 30 mg/kg resveratrol daily by gavage. A A schematic timeline of model mice. B Sirius red staining was applied to assess the degree of liver fibrosis in mice (N = 5, scale bar = 50 µm), one-way ANOVA with Tukey’s post-hoc. C, D The expressions of α-SMA and Collagen I (common markers for liver fibrosis) in mouse liver tissues were measured using immunohistochemical analysis (N = 5), one-way ANOVA with Tukey’s post-hoc. E, F The levels of (E-I) alanine aminotransferase (ALT), (E-II) aspartate aminotransferase (AST), (E-III) alkaline phosphatase (ALP), (F-I) indirect bilirubin (IBil), (F-II) total bilirubin (TBil) and (F-III) direct bilirubin (DBil) in mouse blood samples were determined using a standard clinical automated analyser, one-way ANOVA with Tukey’s post-hoc or Brown-Forsythe and weltch ANOVA with Games-Howell’s post-hoc. N = 5. G Analysis of the hydroxyproline (Hyp) level using a commercial kit, one-way ANOVA with Tukey’s post-hoc. N = 5. H The expression of ZO-1 (intercellular adhesion protein) in mouse colon tissues was measured by immunofluorescence staining (scale bar = 100 µm), one-way ANOVA with Tukey’s post-hoc. N = 5. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 vs. Control or CCl4 group. Col I: Collagen I. Data are presented of three independent assays

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