Fig. 3

Regulation of Staphylococcus_xylosus and Staphylococcus_lentus on the process of liver fibrosis in mice. The pure cultures (10⁹ CFU) of the three kinds of gut microbiota (Staphylococcus_lentus, Aerococcus_viridans and Staphylococcus_xylosus) were given to mice by gavage to make them become the dominant microflora in the intestine, and then the liver fibrosis model of mice was induced by the intraperitoneal injection of CCl₄ (0.5 µL/g). A, B Sirius red staining and immunohistochemical assays were conducted to assess the degree of liver fibrosis in mice (N = 10, scale bar = 50 µm), Brown-Forsythe and Welch ANOVA with Games-Howell’s post-hoc. C Different detection kits were applied to measure the concentrations of ALT, AST and ALP in mouse blood samples, Brown-Forsythe and Welch ANOVA with Games-Howell’s post-hoc. N = 10. D The concentrations of IBiL, TBiL and DBiL in mouse blood samples were assessed, one-way ANOVA with Tukey’s post-hoc for IBil, Brown-Forsythe and Welch ANOVA with Games-Howell’s post-hoc for TBil and DBil. N = 10. E Comparison of the hydroxyproline (Hyp) level using a commercial kit. N = 10. **P < 0.01, ****P < 0.0001 vs. Control. ^#P < 0.05, ^##P < 0.01, ^###P < 0.001, ^####P < 0.0001 vs. CCl₄. S_xylosus: Staphylococcus_xylosus, S_lentus: Staphylococcus_lentus, A_viridans: Aerococcus_viridans. Data are presented of three independent assays