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Fig. 4 | Molecular Medicine

Fig. 4

From: Resveratrol ameliorates liver fibrosis induced by nonpathogenic Staphylococcus in BALB/c mice through inhibiting its growth

Fig. 4

Influence of Staphylococcus_xylosus and Staphylococcus_lentus in the occurrence of BT. The pure cultures (109 CFU) of the two kinds of gut microbiota (Staphylococcus_lentus and Staphylococcus_xylosus) were given to mice by gavage to make them become the dominant microflora in the intestine, and then the liver fibrosis model of mice was induced by intraperitoneal injection of CCl4 (0.5 µL/g). A After bacterial colony culture on mesenteric lymph nodes (MLNs), the occurrence of the colonies was observed. B Statistical analysis on the number of bacterial colonies, one-way ANOVA with Tukey’s post-hoc. N = 10. C Analysis of the concentration of LBP in the mouse blood samples by a commercial kit. N = 10. D The occurrence of BT was assessed using EUB338 FISH detection. N = 10. E The mRNA levels of zonula occludens-1 (ZO-1) and occludin were measured by qRT-PCR. N = 10. F The relative ZO-1 positive areas were measured using immunofluorescence (scale bar = 50 µm), one-way ANOVA with Tukey’s post-hoc. N = 10. **P < 0.01, ***P < 0.001, ****P < 0.0001 vs. Control. #P < 0.05, ##P < 0.01 vs. CCl4. Data are presented of three independent assays

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