Fig. 3

Validation of differentially regulated targets of CREB1 between normal and cancer. A TRIM15, NHERF1, RBPMS2, FERMT2 and FAM20C, each gene expression was measured by qRT-PCR in GES-1 and NCI-N87 cells transfected with CREB1 specific siRNA or CREB1 lentivirus (n = 3–4). B Overexpression or downregulation of CREB1 in GES-1 and NCI-N87 cells, and the expression changes of TCEAL2 and MBNL1 were measured by qRT-PCR (n = 3–4). C CREs in the promoters of TCEAL2 and MBNL1. Sequence analysis of the promoters of the two genes indicated the potential binding sites for CREB1. D TCEAL2 or MBNL1 pGL3 luciferase reporter was co-transfected with CREB1 into 293 T cells and NCI-N87 cells, and relative luciferase activity was detected. *Means significant P-value < 0.05, *** means significant P-value < 0.001, NS means no significant, two-sided Student’s t-test