Fig. 5

2′,3′,4′-THC acts differently from SERMs on gene transcription. A KRT19 and B NKG2E gene expression in U2OS-ERα cells treated with 10 nM E2 or 5 μM 2′,3′,4′-THC in the absence and presence of 1 μM tamoxifen or 1 μM raloxifene alone or in combination for 24 h. The mRNA levels were determined by qRT-PCR. C U2OS cells cotransfected with ERα and NKG2E-TK-Luc were treated with 10 nM E2, 5 μM 2′,3′,4′-THC, 5 μM tamoxifen, and 1 μM raloxifene alone or in combination for 24 h. Luciferase activities were measured with a luminometer. D ERα recruitment to the KRT19 ERE in U2OS-ERα cells was determined with ChIP assay after the cells were treated with 10 nM E2 and 5 μM 2′,3′,4′-THC alone or in combination for 2 h. The KRT19 gene was used because the ERE was previously characterized and the ERE in the reprogrammed genes is not known. The data shown are the mean of triplicate samples ± SE. The statistical significance was determined by one-way ANOVA followed by Tukey’s multiple comparisons post hoc test