Fig. 7

A Representative images of GFAP-labeling (red) in the dentate gyrus of the hippocampus. Hoechst was used to stain the nuclei (blue). Scale bar: 100 µm (n ≥ 15). B Calculation of differences in fluorescence intensity for GFAP. Relative intensity quantification numbers were obtained under the following formula: CTCF (Corrected Total Cell Fluorescence) = Integrated Density − (Area of selected cell X Mean fluorescence of background readings). Differences between groups were analyzed using ANOVA and Tukey’s. Significance was represented as follows: *p < 0.05, **p < 0.01 and ***p < 0.001 (n = 4/experimental group)