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Fig. 5 | Molecular Medicine

Fig. 5

From: Gut microbiota-testis axis: FMT improves systemic and testicular micro-environment to increase semen quality in type 1 diabetes

Fig. 5

A10-FMT improved spermatogenesis process. a IHF staining of testicular germ cell marker VASA, meiosis marker SYCP3, sperm protein PGK2, and transition protein 1 (TP1) in each treatment. Scale bar: 50 μm. n ≥ 3/group. b Quantitative data for VASA IHF staining. The y-axis represents the percentage of total cell. The x-axis represents the treatment. *p < 0.05. c Quantitative data for SYCP3 IHF staining. The y-axis represents the percentage of total cell. The x-axis represents the treatment. *p < 0.05. d Quantitative data for TP1 IHF staining. The y-axis represents the percentage of total cell. The x-axis represents the treatment. *p < 0.05. e Quantitative data for PGK2 IHF staining. The y-axis represents the percentage of total cells. The x-axis represents the treatment. *p < 0.05. f Western blotting analysis of the proteins of important genes for spermatogenesis in each treatment. n ≥ 3/group. g Quantitative data for Western blotting analysis. h IHF staining of Sertoli cell marker SOX9

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