Fig. 2
From: ATF1/miR-214-5p/ITGA7 axis promotes osteoclastogenesis to alter OVX-induced bone absorption
MiR-214-5p is transcriptionally activated by ATF1. Knockdown or overexpression of ATF1 were performed in RAW264.7 cells, ATF1 (A) and miR-214-5p (B) expression in each group were detected via qRT-PCR. C Two predicted binding sites by JASPAR. D ATF1 and miR-214-5p promoter binding site mutation sequence. E, F Luciferase reporter assay in cells transfected with a plasmid containing miR-214-5p-WT, Site 1-MUT and Site 2-MUT. Sh-ATF1 and ATF1 overexpression vector were administrated, respectively. G ChIP assays was conducted in RAW264.7 cells. qRT-PCR was utilized to examine miR-214-5p promoter expression associated with ATF1. Experiments were performed for at least 3 times. Data are presented as mean ± SD. *P < 0.05, **P < 0.01 and ***P < 0.001