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Fig. 3 | Molecular Medicine

Fig. 3

From: ATF1/miR-214-5p/ITGA7 axis promotes osteoclastogenesis to alter OVX-induced bone absorption

Fig. 3

ATF1 inhibition abolishes osteoclast formation, which is reversed by miR-214-5p upregulation. RAW264.7 cells were administrated with sh ATF1/sh NC with or without miR-214-5p mimic/mimic NC. A Expression of ATF1, miR-214-5p and ITGA7 was detected by qRT-PCR. B Protein level of ITGA7 was detected by western blotting. C Cell viability was evaluated by CCK-8 assay. D Cell apoptosis was assessed by flow cytometry. E Expression of osteoclast markers was evaluated by western blotting. F TRAP staining results in each group. Experiments were performed for at least 3 times. Data are presented as mean ± SD. *P < 0.05, **P < 0.01 and ***P < 0.001

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