Fig. 5
From: ATF1/miR-214-5p/ITGA7 axis promotes osteoclastogenesis to alter OVX-induced bone absorption
MiR-214-5p downregulation inhibits osteoclastogenesis and ITGA7 knockdown reverses this trend. RAW264.7 cells were treated with miR-214-5p mimic/mimic NC with or without sh ITGA7/sh NC. A Expression of miR-214-5p and ITGA7 was examined by qRT-PCR. B Protein level of ITGA7 was examined by western blotting. C Cell viability was evaluated by CCK-8 assay in each group. D Cell apoptosis was assessed by flow cytometry. E Expression of osteoclast markers was evaluated by western blotting. F TRAP staining results in each group. Experiments were performed for at least 3 times. Data are presented as mean ± SD. *P < 0.05, **P < 0.01 and ***P < 0.001