Fig. 6
From: ATF1/miR-214-5p/ITGA7 axis promotes osteoclastogenesis to alter OVX-induced bone absorption
MiR-214-5p inhibition prevents OVX-induced osteoporosis. OVX or sham-operated mice were injected with miR-214-5p antagomir/antagomir NC. A Expression of miR-214-5p and ITGA7 was examined by qRT-PCR. B Protein level of ITGA7 was examined by western blotting. C Bone mineral density (BMD, g/cc), trabecular number (Tb.N, 1/mm) and trabecular thickness (Tb.Th, μm) of each sample were investigated by Micro CT. D Expression of osteoclast markers was evaluated by western blotting. E, F Bone tissue sections were exposed to H&E and TRAP staining. G, H Number of osteoclasts per bone surface (N.Oc/BS) and osteoclast surface per bone surface (Oc.S/BS) of mice obtained. N = 6. Data are presented as mean ± SD. *P < 0.05, **P < 0.01 and ***P < 0.001