Fig. 1

Sox10 and EGF act synergistically to transdifferentiate astrocytes into iOPCs. A Representative images of astrocyte purifying. (A1) Primary glial culture of mouse spinal cord tissue. (A2) The cells shown in A1 were passaged to uncoated dishes and cultured for 30 min, astrocytes quickly adhered to the surface of dishes, while other cells were still floating (yellow arrows). (A3) The floating cells were rinsed and astrocytes were retained. (A4) Astrocyte morphology 24 h after purification. B Purified cells were immunoreactive to GFAP and EGFR antibodies. C Representative images of the astrocyte transdifferentiation (15 dpi) in the five cultures. D Quantifications of experiments presented in C. E MBP⁺ mature OLs derived from iOPCs when cultured in the differentiation medium. F Confocal image of myelinated axon in iOPC/DRG co-culture. Purified iOPCs matured into MBP⁺ OLs in the co-culture, and aligned with axons marked by neurofilament (NF-1) staining. Statistical analyses are presented as Mean ± SD, n = 3. ***P < 0.001. Scale bars, A–C 50 μm; E 25 μm; F 10 μm