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Fig. 2 | Molecular Medicine

Fig. 2

From: EGF signaling promotes the lineage conversion of astrocytes into oligodendrocytes

Fig. 2

EGF promotes astrocyte dedifferentiation. A Immunostaining of cells with GFAP, Nestin and Olig2 antibodies 5 days after transdifferentiation. B Quantification of immune-positive cells in the four cultures. C Western blotting analysis of GFAP, Nestin and Olig2 on d5 during the transdifferentiation process. DF Quantitative analysis of GFAP, Nestin and Olig2 proteins expression in the four cultures on d5, respectively, histograms express results in arbitrary units, taking Ctrl cells values as 100%. G Quantitative RT-PCR analysis of the expression level of GFAP, Glast, NFIA and Sox9 in the four groups on d5 during the transdifferentiation process and Sox10 + EGF + Gefitinib group cells treated with FBS for another 3 days. Statistical analyses are presented as Mean ± SD, n = 3. **P < 0.01, ***P < 0.001. Scale bars, 50 μm

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