Fig. 2From: NDR1 increases NOTCH1 signaling activity by impairing Fbw7 mediated NICD degradation to enhance breast cancer stem cell propertiesNDR1 is critical for the regulation of cancer stem cell properties in breast cancer cells. a, b NDR1 siRNA or control siRNA were transfected in SUM149 or MCF-7 cells for 72 h. Cells were harvested and subjected to CD24/44 staining and flow cytometry analysis. c, d NDR1 or control vector were expressed in SUM149 or MCF-7 cells for 72 h. Cells were harvested and subjected to CD24/44 staining and flow cytometry analysis. e, f NDR1 siRNA or control siRNA were transfected in SUM149 or MCF-7 cells for 72 h. Cells were harvested and subjected to ALDEFLUOR staining and flow cytometry analysis. g, h NDR1 or control vector were expressed in SUM149 or MCF-7 cells for 72 h. Cells were harvested and subjected to ALDEFLUOR staining and flow cytometry analysis. i, j SUM149 or MCF-7 cells expressed NDR1 shRNA or control vector were subjected to sphere-forming assay. The representative spheres were shown in i. The distribution of sphere diameters was shown in j. k, l SUM149 or MCF-7 cells expressed NDR1 or control vector were subjected to sphere-forming assay. The representative spheres were shown in k. The distribution of sphere diameters was shown in l. The bar represents mean ± SD of three independent experiments (*: p < 0.05, **: p < 0.01, ***: p < 0.001)Back to article page