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Fig. 3 | Molecular Medicine

Fig. 3

From: Famotidine activates the vagus nerve inflammatory reflex to attenuate cytokine storm

Fig. 3

The mast cell and macrophage are not targets for famotidine. AF Mast cell deficient KitW−sh/KitW−sh sash mice had similar LPS-induced TNF, IL-6, IL-1β response as wild type mice. Male wild type (WT) or “sash” mice received LPS injection (IP, 7 mg/kg) and were euthanized 2.5 h post-LPS injection. Serum and spleen TNF, IL-6 and IL-1β were measured. N = 3. GJ Famotidine, administered ICV, reduced LPS-induced TNF and IL-6 release in mast cell deficient KitW−sh/KitW−sh sash mice at 2.5 h post LPS exposure. Male KitW−sh/KitW−sh sash mice received famotidine or PBS (0.4 mg/kg, in 5 µl volume) administered ICV at 30 min prior to LPS injection (IP, 7 mg/kg). Mice were euthanized 2.5 h post-LPS injection and serum TNF and IL-6 were measured. N = 3 for normal wild type, n = 4 for normal “sash”, 5 for LPS, 5 or 7 for LPS + famotidine group. *P < 0.001. **P < 0.0001. KN RAW 264.7 cells (K-L) or thioglycollate-elicited mouse primary peritoneal macrophages (M–N) in 96-well culture plates were stimulated with LPS (0.4 ng/ml) in combination with various amounts of famotidine for 16 h. TNF (K,M) and IL-6 (L,N) released in the supernatants were measured. n = 3–6 per treatment. *P < 0.05 vs. LPS alone

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