Fig. 4

Effect of hypoxia on oxidation indexes in OA-FLSs and RA-FLSs. A Cells were exposed to 21% or 2% O₂ for 24 h and assessed the ROS content by flowcytometry using 10 μM CM-H2DCFDA as a ROS probe; B JC-1 was used to detect the effect of hypoxia on MMP of OA-FLSs and RA-FLSs; C Histogram depicts the quantitative data from the JC-1 staining (mean ± SD, n = 6 from three independent experiments); D The expressions of HIF-1α and BNIP3 in OA-FLSs and RA-FLSs after hypoxia for 12 h were analyzed by Western blot (mean ± SD, n = 6 from three independent experiments); E The expressions of HIF-1α and BNIP3 in OA-FLSs and RA-FLSs after hypoxia for 12 h were analyzed by RT-qPCR (mean ± SD; n = 6 from three independent experiments); F The expressions of HIF-1α and BNIP3 in OA-FLSs and RA-FLSs after hypoxia for 24 h were analyzed by Western blot (mean ± SD; n = 6 from three independent experiments); G The expressions of HIF-1α and BNIP3 in OA-FLSs and RA-FLSs after hypoxia for 24 h were analyzed by RT-qPCR (mean ± SD; n = 6 from three independent experiments). ^#P < 0.05, ^##P < 0.01 represents the comparison between OA-FLSs N and RA-FLSs N; *P < 0.05, **P < 0.01 represents the comparison between OA-FLSs N and OA-FLSs H, ⁺P < 0.05, ⁺⁺P < 0.01 represents the comparison between RA-FLSs N and RA-FLSs H, ^ΔP < 0.05, ^ΔΔP < 0.01 represents the comparison between OA-FLSs H and RA-FLSs H