Fig. 3

HCQ induces the apoptosis of BM-derived MDSCs under stimulation with R848 and reverses the expression of CD81 in MDSCs. A The scheme showing the experimental design. Cells from bone marrow (BM) were stimulated by GM-CSF (40 ng/mL) and IL-6 (40 ng/mL) for 4 days, then, treated with or without R848 (100 ng/mL) and HCQ (2, 6, 20 µg/mL), and after 24 h, the cells were harvested for detection. B, C. The proportions (B) and apoptotic rate (C) of bone marrow-derived CD11b⁺Gr-1⁺ MDSCs were determined by FACS (n = 4). D The percentages of CD81⁺ in MDSCs were determined by FACS in BM-derived MDSCs (C) (n = 4). E Western blot was used to detect the expression of CD81 protein in BM-derived MDSCs (n = 4). Data are presented as the mean ± standard error of the mean (SEM). *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001