Fig. 6

A Relative gene expression levels of inflammation-related factors (IKKɛ, SHIP1, TNF-α, FOXO3A, IL-1β, IL-6, PI3K, IFN-γ, AKT, and NF-κB) 72 h after treatment of HUVECs with NFs (M5) as determined by RT-qPCR. B Western blot analysis of IL-1β protein expression in cells treated with 0.1 μg/mL LPS or NF-M5 for 72 h. C Relative IL-1β protein levels (expression levels were normalized to that of GAPDH). D Western blot analysis of SHIP1 protein expression in cells treated with 0.1 μg/mL LPS or NF-M5 for 72 h. E Relative SHIP1 protein levels (expression levels were normalized to that of GAPDH)