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Table 2 Template and primer sequence for preparing nucleic acid nanoflowers that can activate miR-155

From: Preparation of a miR-155-activating nucleic acid nanoflower to study the molecular mechanism of miR-155 in inflammation

Name

Sequence (5′ → 3′)

Template-1

ATAGTGAGTCGTATTAACGTACCAACAAGTCACCTCAGCCTCCCAAAACTTGTTTGGGAGGCTGAGGTGACATCCCT

Template-2

ATAGTGAGTCGTATTAACGTACCAACAATATCCCTCTTAGTCTGCTAACTTGTAGCAGACTAAGAGGGATAATCCCT

Template-3

ATAGTGAGTCGTATTAACGTACCAACAAGTCTGCTAGGGTTGCCATAACTTGTATGGCAACCCTAGCAGACATCCCT

Template-4

ATAGTGAGTCGTATTAACGTACCAACAAATAGACTGGATGGCTGATAACTTGTATCAGCCATCCAGTCTATATCCCT

Template-5

ATAGTGAGTCGTATTAACGTACCAACAAACATTCTGGAGGCTAGAAAACTTGTTTCTAGCCTCCAGAATGTATCCCT

Template-6

ATAGTGAGTCGTATTAACGTACCAACAAGGACTCTCTTCCTGGCTTAACTTGTAAGCCAGGAAGAGAGTCCATCCCT

Template-7

ATAGTGAGTCGTATTAACGTACCAACAATCTTCCTGGCTTACAGGAAACTTGTTCCTGTAAGCCAGGAAGAATCCCT

T7

TAATACGACTCACTATAGGGAT

  1. All the above sequences (except T7) need to modify the phosphate group at the 5′end
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Molecular Medicine

ISSN: 1528-3658