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Fig. 1 | Molecular Medicine

Fig. 1

From: Insulin-inducible THRSP maintains mitochondrial function and regulates sphingolipid metabolism in human adipocytes

Fig. 1

Insulin induces THRSP expression in human adipose tissue and human and mouse adipocytes. a Subcutaneous adipose tissue (SAT) biopsies were obtained in a euglycemic hyperinsulinemic clamp and THRSP mRNA expression was measured at basal, 180 min and 360 min (n = 36). b THRSP expression correlated with M-value, which is a measure of insulin sensitivity. The correlation was the strongest at 360 min. c Subjects were divided to insulin sensitive (IS; n = 18) and insulin resistant (IR; n = 18) group by median of M-value. THRSP induction by insulin was higher in IS group in comparison to IR group. d Correlation coefficients of waist-to-hip ratio, amount of SAT, fasting plasma glucose (fP-glucose), fasting serum insulin (fS-insulin) and C-peptide (C-pep) e Insulin induced THRSP protein expression in SGBS adipocytes (n = 6, three independent experiments). f LY294002, an inhibitor of PI3K, blocked insulin-mediated THRSP induction, measured at mRNA level (n = 6, two independent experiments). g Insulin induced THRSP protein expression in 3T3-L1 adipocytes (n = 10, three independent experiments). h Insulin induced THRSP mRNA expression in 3T3-L1 adipocytes (n = 5, two, independent experiments). The data is represented as mean with SD. Statistical significance is designated as *P ≤ 0.05 **P < 0.01, ***P < 0.001, ****P < 0.0001

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