Fig. 3
From: ISM1 suppresses LPS-induced acute lung injury and post-injury lung fibrosis in mice
Increase of AMs in Ism1−/− mouse under LPS is mainly due to increase of Mo-AMs. BALF of 8–9 weeks old WT and Ism1−/− mice was collected at day 3 and day 5 after intratracheal administration of 2 mg/kg LPS (n = 4–5). After BALF collection, cells were isolated, erythrocytes were lysed, and stained with Viobility fixable dye for live/dead cells, various marker antibodies and Annexin-V and analyzed by flow cytometry. Total cells from BALF were presented in panel a-d. The flow cytometry gating strategy is shown in Additional file 1: Fig. S5. a Quantification of the amount of TR-AM and Mo-AM. b Percentage of early apoptotic Mo-AM as identified as Annexin-V+ in Mo-AM population. c Percentage of early apoptotic TR-AM as identified as Annexin-V+ cells in TR-AM population. d Percentage of early apoptotic neutrophils as identified as Annexin-V+ cells in neutrophil population. e Representative histograms showing the percentage of Annexin-V+ cells in Mo-AM, RT-AM, and neutrophil population on day 5 post LPS. Statistical analysis was conducted by Student's t-test. “*” represents P < 0.05; “***” represents P < 0.001