Fig. 3

SCs-Exo activates macrophages into a proregenerative phenotype. A Schematic representation of an in vitro coculture system for assessing the role of SCs in regulating macrophage function. B Transwell migration assay of U937 cells cocultured with SCs for 48 h in the presence or absence of lncARAT overexpression. C IF analysis of CD206 in injured nerves 4 dpi after lncARAT knockdown. Scale bar, 50 µm. D and E Western blot and quantitative analysis of IL-10, CD206, and Arg1 expression in macrophages cocultured with SCs for 48 h in the presence or absence of lncARAT overexpression. F qRT-PCR analysis of lncARAT expression in SCs-Exo/cont and SCs-Exo/Lv-lncARAT. G qRT-PCR analysis of IL-10, CD206, and Arg1 expression in macrophages after treatment with SCs-Exo/Lv-cont or SCs-Exo/Lv-lncARAT for 48 h. H and I Western blot and quantitative analysis of IL-10, CD206, and Arg1 expression in macrophages after treatment with SCs-Exo/Lv-cont or SCs-Exo/Lv-lncARAT for 48 h. J IF and quantitative analysis of CD206 expression in macrophages after treatment with SCs-Exo/Lv-lncARAT in the presence or absence of GW4869 (exosome inhibitor, 10 µM). Scale bar, 50 µm. *p < 0.05. **p < 0.01