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Fig. 4 | Molecular Medicine

Fig. 4

From: Upregulated lncARAT in Schwann cells promotes axonal regeneration by recruiting and activating proregenerative macrophages

Fig. 4

LncARAT is transferred from SCs to macrophages via SCs-Exo. A qRT-PCR analysis of lncARAT expression in SCs-Exo after RNase treatment in the presence or absence of Triton X-100. B Fluorescence microscopy analysis of U937 macrophages treated with PKH67 (green)-labeled SCs-Exo. PBS served as a negative control. Scale bar, 20 µm. qRT-PCR analysis of lncARAT expression in SCs (C) and SCs-Exo (D) after lncARAT overexpression. E qRT-PCR analysis of lncARAT expression in U937 macrophages treated with SCs-Exo/Lv-cont or SCs-Exo/Lv-lncARAT. F Fluorescence microscopy analysis of U937 macrophages treated with SCs-Exo/FAM-lncARAT. Scale bar, 20 µm. GI qRT-PCR analysis of IL-10, CD206, and Arg1 expression in U937 macrophages after treatment with Lv-cont or Lv-lncARAT for 48 h. J and K Western blot and quantitative analysis of IL-10, CD206, and Arg1 expression in U937 macrophages after treatment with Lv-cont or Lv-lncARAT for 48 h. L IF assay of CD206 expression in cross sections of sciatic nerves at 4 dpi from sham rats or rats with sciatic nerve injury after Lv-lncARAT knockdown. Scale bar, 100 µm. *p < 0.05. **p < 0.01

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