Fig. 5

LncARAT sponges miRNA-329-5p to induce macrophages activation into a proregenerative phenotype. A Subcellular localization analysis of lncARAT in U937 macrophages using RNA-FISH analysis. Scale bar, 20 µm. B Biotinylated miRNAs were transfected into U937 macrophages, and then an RNA pull-down assay was performed to assess the interaction of lncARAT with specific miRNAs. C Biotinylated miRNA-329-5p mutants were transfected into U937 macrophages, and then an RNA pull-down assay was performed. D Biotinylated lncARAT was transfected into U937 macrophages, and then an RNA pull-down assay was performed to assess miR-329-5p enrichment. E Schematic representation of the miRNA-329-5p site in lncARAT. F Luciferase activity was assayed in HEK293 cells after cotransfection with miRNA-329-5p and luciferase reporters containing lncARAT. G RIP assays with anti-Ago2 antibody were performed to assess the enrichment of lncARAT and miRNA-329-5p in U937 macrophages. RIP assays with anti-Ago2 antibody were performed to assess the enrichment of miRNA-329-5p (H) and lncARAT (I) in response to miRNA-329-5p inhibition. J qRT-PCR analysis of miRNA-329-5p expression in U937 macrophages after lncARAT overexpression or knockdown. Western blot (K) and quantitative analysis of L IL-10, CD206, and Arg1 expression in U937 macrophages after Lv-lncARAT overexpression in the presence or absence of miRNA-329-5p. *p < 0.05. **p < 0.01