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Fig. 3 | Molecular Medicine

Fig. 3

From: FGF1 alleviates LPS-induced acute lung injury via suppression of inflammation and oxidative stress

Fig. 3

FGF1 pretreatment dampens LPS-induced inflammatory cell infiltration and cytokine production. A Inflammatory cell infiltration was quantified by counting the total number of inflammatory cells, neutrophils, and macrophages in BALF using the Wright-Giemsa method (red arrows indicate the neutrophils and black arrows indicate the macrophages). Scale bar: 64 μm, magnification: 40×. B Representative immunofluorescence analysis of A549 cells for TNF-α expression, (scale bar = 64 μm, magnification: 10×). C WB analysis and quantification of the relative protein expression of murine IL-6, IL-1β, and TNF-α; β-actin was used for normalization. Data are presented as mean ± SD (n = 3 to 5 per group). *p < 0.05, **p < 0.01; One-way ANOVA with Tukey’s post-hoc test

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