Fig. 4

FGF1 pretreatment inhibits NF-κB nuclear translocation. A Immunofluorescence analysis and quantification of NF-κB p65 nuclear expression in A459 cells across different groups (scale bar = 64 μm). The fluorescence levels were quantified using ImageJ software. Corrected Total cellular Fluorescence (CTCF) = integrated density (area of selected region × mean fluorescence of background readings). B The relative protein expression levels of nuclear NF-κB p65, Lamin B, IκBα, p-IκBα were analyzed by western blot. Bar graphs show the quantification of protein expression levels normalized to GAPDH. All data were analyzed at 12 h after LPS treatment. Data are presented as mean ± SD (n = 3 to 5 per group). **p < 0.01, ***p < 0.001; One-way ANOVA with Tukey’s post-hoc test