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Fig. 5 | Molecular Medicine

Fig. 5

From: FGF1 alleviates LPS-induced acute lung injury via suppression of inflammation and oxidative stress

Fig. 5

The effects of FGF1 pretreatment of LPS-induced cell death. A Representative images of TUNEL assay staining to detect apoptotic cells in the lung sections of mice at 12- and 24 h. Scale bar = 100 μm. Apoptotic cells were observed under fluorescence microscopy and the percentage of TUNEL positive cells per 40x magnification field was calculated. B WB analysis and quantification of TLR4 and the downstream apoptosis-related protein cleaved-caspase 3 in murine lungs. WB assays were performed at 12 h post LPS treatment. Data are presented as mean ± SD (n = 3 per group). **p < 0.01, ***p < 0.001, ****p < 0.0001; One-way ANOVA with Tukey’s post-hoc test

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