Skip to main content

Table 1 Primer sequences for the real-time polymerase chain reaction of the genes associated osteoblast differentiation

From: Probucol promotes osteoblasts differentiation and prevents osteoporosis development through reducing oxidative stress

Target gene

Forward primer (5ʹ-3ʹ)

Reverse primer (5ʹ-3ʹ)

Runx2

GCCGTAGAGAGCAGGGAAGAC

CTGGCTTGGATTAGGGAGTCAC

OC

CGGCAAGGCTTCGCATCTG

GGAGCAGAGCAGACAGGTGAACT

ALP

AGCGACACGGACAAGAAGC

GGCAAAGACCGCCACATC

SOD1

GGTGGGCCAAAGGATGAAGAG

CCACAAGCCAAACGACTTCC

SOD2

GGGGATTGATGTGTGGGAGCACG

AGACAGGACGTTATCTTGCTGGGA

CAT

TGG GAT CTC GTT GGA AAT AACAC

TCA GGA CGT AGG CTC CAG AAG

GAPDH

AGAAAAACCTGCCAAATATGATGAC

TGGGTGTCGCTGTTGAAGTC

  1. Superoxide dismutase 1 and 2 (SOD1, 2); Glyceraldehyde 3-phosphate dehydrogenase (GAPDH); Osteocalcin (OC); RUNX2 (core binding factor alpha 1, core binding factor a1); Catalase (CAT)
Back to article page

Molecular Medicine

ISSN: 1528-3658