Fig. 3

Schematic representation of potential regulatory mechanisms of HDAC6 by lncRNA H19. A Differentiated C2C12 cells were transfected with either the scramble or H19 siRNA (5 nM) and after 48 h, total RNA was isolated and the levels of miR-675-5p and miR-675-3p were assessed by qRT-PCR. Sno-234 was used as the loading control. Data are means of at least three independent experiments and values are means ± SD. *p ≤ 0.05, ***p ≤ 0.001. B Potential binding between the lncRNA H19 and HDAC6 mRNA as derived from the online LncRRIsearch prediction tool. UTR: Untranslated Region; CDS: Coding DNA sequence. C Binding of H19 to its interactors might prevent their occupancy on the HDAC6 promoter, thereby preventing its transcription. In the absence or decreased levels of H19, the interactors get free to bind to their potential binding elements on the HDAC6 promoter to increase HDAC6 gene transcription