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Table 1 Primer sequences used for qRT-PCR and cloning

From: H19 inhibition increases HDAC6 and regulates IRS1 levels and insulin signaling in the skeletal muscle during diabetes

Gene name

Primer sequence [5′–3′]

H19

Forward: CCTCAAGATGAAAGAAATGGTGCTA

 

Reverse: TCAGAACGAGACGGACTTAAAGAA

Hdac1

Forward: GACGGCATTGACGACGAATC

 

Reverse: TGAAGCAACCTAACCGGTCC

Hdac2

Forward: GGAATGTTGCCCGATGTTGG

 

Reverse: TGCAGTTTGAAGTCTGGTCC

Hdac3

Forward: TTCCCTCAAACTCTCACGGC

 

Reverse: AGTTGCTGGGGCTCATTACC

Hdac4

Forward: CCCGCTACTGGTATGGGAAG

 

Reverse: GGTGGTTGTAGGAGGCTGAC

Hdac5

Forward: GAGGGAGGCCATGACTTGAC

 

Reverse: TCTCTAGTGTGGCAACCGC

Hdac6

Forward: ACTGCACTGCCCATGGATAC

 

Reverse: GTAGGATGAGACAGCGAGCC

Prkab2

Forward: TCACCTACCTCCTGGCTCTC

 

Reverse: AGAGTCTCAGCTGAGGGAGG

Pfkfb3

Forward: GTCGCCGAATACAGCTACGA

 

Reverse: GAGCCCCACCATCACAATCA

Socs2

Forward: CAGCTGGACCGACTAACCTG

 

Reverse: GGTGAACAGTCCCATTCCGT

Srebf1

Forward: TAGTGACTCTGAGCCCGACA

 

Reverse: ATCTCTGCTCTCTGCCTCCA

Irs1

Forward: TGGACATCACAGCAGAATGAAGA

 

Reverse: GACGTGAGGTCCTGGTTGTG

Ppp2r5b

Forward: TTGACCCGTGTTGTCCACTG

 

Reverse: AGGCTGGCACATCTTTGAGC

18S rRNA

Forward: AGAAACGGCTACCACATCCA

 

Reverse: CCCTCCAATGGATCCTCGTT

miR-675-5p StemLoop

CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGACTGTGGG

miR-675-5p FP

Forward: ACACTCCAGCTGGGTGGTGCGGAAAGGGCC

miR-675-3p StemLoop

CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGACTGAGCG

miR-675-3p FP

Forward: ACACTCCAGCTGGGCTGTATGCCCTAACC

Universal RP for miR-675-5p/3p

Reverse: CTCAATCGTACATAGAAACAGGGATC

Sno-234 StemLoop

GGATCGCCTCTCAGTGGTAG

Sno-234

Forward: GGCTTTTGGAACTGAATCTAAGT

Reverse: GAGGTATTCGCACCAGAGGA

HDAC6 Cloning primers

Forward Kpn1 site: CGGGGTACCATGACCTCCACCGGCCAAGATTC

Reverse EcoR1 site: CCGGAATTCGTGTGAGTGGGGCATGTCCTC

  1. FP forward primer, RP reverse primer
  2. Inserted restriction enzyme sites are indicated in bold and underlined letters in the HDAC6 cloning primers
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Molecular Medicine

ISSN: 1528-3658