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Fig. 2 | Molecular Medicine

Fig. 2

From: Recombinant truncated latency-associated peptide alleviates liver fibrosis in vitro and in vivo via inhibition of TGF-β/Smad pathway

Fig. 2

Purification and identification of Recombinant LAP and tLAP. A Purification of recombinant tLAP. lane M, protein marker; lane 1, Crushing bacterium fluid; lane 2, Supernatant; lane 3, Precipitation after crushing; lane 4, wash; lane 5–8, elution for 4 times. B Purification of recombinant LAP. lane M, protein marker; lane 1, Crushing bacterium fluid; lane 2, Supernatant; lane 3, Precipitation after crushing; lane 4, wash; lane 5–8, elution for 4 times. C Identification of recombinant tLAP by pull down assay. M, protein marker; lane 1, LAP + Ni–NTA agarose; lane 2, tLAP + Ni–NTA agarose; lane 3, TGF-β1; lane 4, TGF-β1 + Ni–NTA agarose; lane 5, TGF-β1 + LAP + Ni–NTA agarose. Lane 6, TGF-β1 + tLAP + Ni–NTA agarose

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Molecular Medicine

ISSN: 1528-3658