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Fig. 3 | Molecular Medicine

Fig. 3

From: FZD7 accelerates hepatic metastases in pancreatic cancer by strengthening EMT and stemness associated with TGF-β/SMAD3 signaling

Fig. 3

PANC-1 was selected for experimentation in vitro, and the effective siRNA was screened by Western blot (A). Silencing FZD7 suppressed PANC-1 cell migration and invasion in Wound healing and Transwell assays (B); whereas FZD7 over-expression promoted PANC-1 cell migration and invasion in Wound healing and Transwell assay (C). In human PDAC, FZD7 gene expression was significantly positively correlated with that of the mesenchymal-related genes, ZEB1, VIM, SNAIL2, with Pearson co-efficients of 0.622 (P = 2.04e−20), 0.593 (P = 2.55e−18), and 0.564 (P = 2.31e−16), respectively, and non-significantly negatively correlated with the epithelial-related gene CDH1 (P = 0.153) (D). In PDAC cells, after the expression of FZD7 was down-regulated by siRNAs, the expression of ZEB1, Vimentin and Slug proteins were all reduced with statistical significance (E). Significant, *P < 0.05, **P < 0.01, ***P < 0.001, Student’s t-test

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