Fig. 6

After treatment of 10 ng/ml TGF-β1 for 48 h, PANC-1 cells acquired a fusiform and elongated morphology, while shFZD7-PANC-1 acquired no morphological change (A). Western blot showed that after the treatment of TGF-β1, Vimentin, Slug, Zeb1, ABCG2 expression were increased, similarly, TGF-β1 could not change these phenotype protein expression levels of shFZD7-PANC-1 (B). CD24⁺CD44⁺ cells population representing CSC subsets were increased after treatment of TGF-β1, when FZD7 expression was silenced, this effect was attenuated (C). Significant, *P < 0.05, **P < 0.01, ***P < 0.001, Student’s t-test