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Fig. 2 | Molecular Medicine

Fig. 2

From: Fn14 exacerbates acute lung injury by activating the NLRP3 inflammasome in mice

Fig. 2

Blockade of Fn14 by ATA alleviates LPS-induced ALI in mice. ATA (20 mg/kg) was administered to mice 1 h before induction of ALI by LPS (5 mg/kg). A, B The protein expression of Fn14 in the lungs was determined by Western blot (n = 4). C The mortality was monitored every 6 h over 120 h (n = 20 per group). C57BL/6J mice were treated with saline or ATA (20 mg/kg). One hour later, mice were given saline or LPS (5 mg/kg, i.t.). D Twelve hours later, the lung histopathological change was determined by H&E staining (Bar = 50 μm). E The change in lung inflammation score was determined (n = 6). F The linear associations between the expression of Fn14 protein and inflammation score were analyzed with Pearson correlations analysis. G The activity of LDH in BALF was determined (n = 6–7). H The lung weight/body weight ratio was determined (n = 6–7). I–K The Quantification of total cells, macrophages, and neutrophils in BALF was determined (n = 6–7). L The activity of MPO in lung tissue was determined (n = 6–10). Data are expressed as the mean ± SD. Differences among multiple groups were performed using ANOVA. Tukey’s test was used as a post hoc test to make pair-wise comparisons. Survival data were analyzed using the log-rank test. *P < 0.05, **P < 0.01, and ***P < 0.001

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