Fig. 5

Blockade of Fn14 by ATA inhibits the NLRP3 inflammasome activation in ALI mice. Adult C57BL/6J mice were treated with saline or ATA (20 mg/kg). One hour later, mice were given saline or LPS (5 mg/kg, i.t.). A–C Twelve hours later, the expression of Asc, Nlrp3, and pro-Il-1β mRNA in the lungs was determined by real-time PCR (n = 3–7). D–G The levels of NLRP3, Caspase-1 p10, and Caspase-1 p20 protein in the lungs were determined by Western blot (n = 6). H–I The content of IL-1β protein in BALF and lung tissue was determined by ELISA (n = 6–8). J The linear associations between the expression of Fn14 protein and IL-1β protein were analyzed with Pearson correlations analysis. Data are expressed as the mean ± SD. Differences among multiple groups were performed using ANOVA. Tukey’s test was used as a post hoc test to make pair-wise comparisons. *P < 0.05, **P < 0.01, and ***P < 0.001