Fig. 1

TRPM3 activity after PregS stimulation. Data were obtained under whole-cell patch-clamp conditions. Comparing all groups, amplitude of ionic current after PregS stimulation we found a significant difference (p = 0.0010). A A representative time-series of current amplitude at + 100 mV and − 100 mV showing the effect of 100 μΜ PregS on ionic currents in isolated NK cells from HC. B I–V before and after PregS stimulation in a cell corresponding with (A). C A representative time-series of current amplitude at + 100 mV and − 100 mV showing the effect of 100 μΜ PregS on ionic currents in isolated NK cells from ME/CFS patients. D. I–V before and after PregS stimulation in a cell as shown in (C). E A representative time-series of current amplitude at + 100 mV and − 100 mV showing the effect of 100 μΜ PregS on ionic currents in isolated NK cells from post COVID-19 condition patient. F I–V before and after PregS stimulation in a cell corresponding with (E). G Bar graphs representing TRPM3 current amplitude at + 100 mV after stimulation with 100 μΜ PregS in HC patients (N = 5; n = 34) compared with post COVID-19 condition patients (N = 5; n = 38) and ME/CFS patients (N = 5; n = 26). TRPM3 currents were determined as a change in amplitude from baseline to PregS induced peak as represented in time-series graphs. I–V curves were used to identify an outward rectification typical of TRPM3. N refers to number of participants and n to number of records analysed. Data are represented as mean ± SEM. HC healthy control, ME/CFS myalgic encephalomyelitis/chronic fatigue syndrome